BS PD ISO/TS 19006:2016

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Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

Category: BS

Standard By:BSI Group, 08/31/2016

Published:2016-08-31

Pages:32

File Size:1 file , 3.1 MB

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BS PD ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.

The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

Cross References:

ISO 10993-5:2009

ISO 29701:2010

ISO/TS 80004-2:2015

ISO/TS 18827

ISO/TR 13097:2013

All current amendments available at time of purchase are included with the purchase of this document.